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KMID : 0381919720020010007
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Korean Journal of Microscopy
1972 Volume.2 No. 1 p.7 ~ p.15
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An Electron Microscopy of Oogenesis and Fertilization in Clonorchis sinensis.
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Abstract
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The morphological and fine structural changes during the oogenesis of Clonorchis sinensiswere studied on the developing ovums in the ovary and ootype with electron microscope. Adult worms were removed from the hepar of the rats which had previously infected with metacercariae of Clonorchis sinensis. The ovary including the Mehlis´ glands and an ootype from adult worm was prefixed for 1-2 hours in 1.25% glutaraldehyde buffered with 0.2M cacodylate at PH 7.2, secondarily fixed for 30 minutes in potassium bicromate and postfixed for an hour in. 1% osmic acid buffered with 0.4M cacodylate at PH 7.2.
After fixation tissues were dehydrated in an alcohol series, embedded in Epon 812 from propylene oxide and stained with saturated uranyl acetate and Pb(N03)2 solution. Material was. examined with a Hitachi HS-7S electron microscope.
The periphery of the ovary, except for the posterior region, is made up of oogonia. As the oogonia divide they proliferate primary oocytes toward the central part of the ovary. After a period of growth the primary oocyte leaves the ovary and is penetrated by a sperm in the ootype Sperm penetration immediately activates the primary oocyte to resume its meiotic activity. After the oocytes meiotic activity is completed, the pronuclei fuse to form a single cleavage nucleus which possesses two nucleoli.
As the oocytes develop their cytoplasmic materials are abundant; small mitochondria are abundant and often their profiles are more numerous in one part of the cytoplasm than elsewhere; the granular endoplasmic reticulum becomes alveolar-sac form and after it leaves the ovary it becomes stratified form.
The reticulate Golgi apparatus is apparent in the developed oocyte.
A little of cortical granules are distributed inside of the plasma membrane in oogonia and large quantity of cortical granules are arranged just inside of the plasma membrane of the primary oocyte and after fertilization they are disappeared with broken out.
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